Journal: Frontiers in Immunology
Article Title: Disrupted balance between pro-inflammatory lipid mediators and anti-inflammatory specialized pro-resolving mediators is linked to hyperinflammation in patients with alcoholic hepatitis
doi: 10.3389/fimmu.2024.1377236
Figure Lengend Snippet: Dysregulated lipid mediators from the AA pathway in AH patients. (A) Simplified schematic representation of the AA metabolic pathway. Key biosynthetic enzymes are shown next to arrows, lipid intermediators in box, and proinflammatory lipid mediators in red and anti-inflammatory lipid in blue. (B–F) Scatter plots showing plasma levels of LTB4 (B, C) , PGD2 (D) , LXA4 (E) , and LTB4/LXA4 ratio (F) in healthy controls (HC), patients with alcoholic hepatitis (AH), and heavy drinking controls (HDC). Concentrations were measured by LC-MS/MS (B; open symbols) or ELISA (C-E; filled symbols). Kruskal-Wallis test with Dunn’s correction for pairwise comparison among AH, HDC, and HC. * p < 0.05, ** p < 0.01, *** p < 0.001. ns, not significant.
Article Snippet: Plasma levels of PGD2, PGE2, LTB4, LXA4, RvE1, RvD2, and MaR1 were quantified using the Prostaglandin D2 ELISA Kit (Cayman Chemical, Ann Arbor, MI), Prostaglandin E2 ELISA Kit (Cayman Chemical, Ann Arbor, MI), LTB4 Parameter Assay Kit (R&D Systems, Minneapolis, MN), Lipoxin A4 ELISA Kit (Neogen, Lexington, KY), Human Resolvin E1 ELISA Kit (MBS025958, MyBioSource, San Diego, CA), Resolvin D2 ELISA Kit (Cayman Chemical, Ann Arbor, MI), and Maresin 1 ELISA Kit (Cayman Chemical, Ann Arbor, MI), respectively.
Techniques: Liquid Chromatography with Mass Spectroscopy, Enzyme-linked Immunosorbent Assay, Comparison